Tetrapyrrole accumulation maintained in <i>papp5crd</i>.

<p>A) Relative fluorescence corresponding to Mg-ProtoIX and Mg-ProtoIX-ME in seedlings from Col0, <i>papp5</i>, <i>crd</i>, and <i>papp5crd</i> during the dark to light transition. 3-d-old seedlings were transferred to constant light (100 µmol photons light cm⁻² sec⁻¹) and samples were collected following 12 h and 24 h exposure. Each data point represents the mean (± SD) of four independent biological replicates. Fluorescence data is complemented with Western blot analysis of the CRD protein levels in the different genotypes. β-Tubulin was used as protein loading control. B) Relative expression of <i>APX2</i> (At3g09640) in Col0, <i>papp5</i>, <i>crd</i>, and <i>papp5crd</i>. Expression levels were compared to the respective dark control for each genotype and relative expression was calculated using Ubiquitin-protein ligase (At4g36800) as a reference gene. Each bar represents the mean (± SD) of at least three independent biological samples. Significant differences relative to Col0 (<i>crd</i> and <i>papp5</i>) and to <i>crd</i> (<i>papp5crd</i>) were calculated according to <i>t-test</i> (a, P<0.001; b, c, P<0.01)