Complement components C3 and C5 attach to ULVWF strings secreted by, and anchored to, stimulated HUVECs.

<p>HUVECs were stimulated and stained as in the legend for Fig. 3, except that antibody to human C3 was used in (A) and antibody to human C5 in (B) to identify complement component attachment to the ULVWF strings. In (A) panel 6, the upper dotted line shows the location of the C3 intensity measurements and the lower dotted line identifies the locations of background intensity measurements. In (B) panel 6, only the locations of the background intensities are identified by the dotted line. The white arrows in (B) panel 3 indicate C5 attachment to the strings. Images were selected from 6 (C3) and 5 (C5) independent experiments.