Construction of the TA-based expression vectors.

<p>A, workflow for the direct cloning of PCR products using the TA-based expression vector system. TA-based expression vectors were digested by XcmI to produce 3′-T-overhangs. The PCR products were ligated with the XcmI-digested vector by T4 ligase. B, Left: Self-ligation of the XcmI-digested vector by T4 ligase. Right: Ligation of the PCR product of the GFP gene with the XcmI-digested vector by T4 ligase, followed by transformation into DH5α strains. C, Samples of restriction digestion analyses of randomly selected colonies from the ligation of the XcmI-digested pGreen vector with the product of the GFP gene using T4 ligase.