RACK1 is involved in the regulation of TGF-β1-mediated signaling pathways in HSCs.

<p>(A, B) Primary mouse HSCs were incubated with lentivirus expressing Myc-RACK1 or control for 96 h. Then cells were treated with TGF-β1 (10 ng/ml) and subjected to western blot analysis. (C, D) Primary mouse HSCs were incubated with lentiviral particles expressing shRNA targeting RACK1 or non-target control for 96 h. Then cells were treated with TGF-β1 (10 ng/ml) and subjected to western blot analysis. (E) Primary HSCs were treated with TGF-β1. Cell lysates were subjected to immuoprecipitation with anti-RACK1 antibody, followed by western blot with related antibodies.