Exiqon miRNA microarray confirms 8 Rapamycin-dependent miRNA.

<p>621-101 cells were treated with Rapamycin 20 nM or DMSO for 24 hours. Total RNA was isolated and applied to the Exiqon platform, which assays 946 human miRNA. <b>A</b>) Heat map of miRNA dysregulated by Rapamycin >1.5-fold, log₂ scale. RNA from three biologic replicates per condition was pooled; each miRNA was assayed in quadruplet on the array. <b>B</b>) miRNA dysregulated by Rapamycin >1.5-fold (normalized to RNU44). Highlighted miRNA (except miR-31 and 210) are common to both the Exiqon and Signosis platforms. miR-21 is circled.