qRT-PCR confirmation of Rapamycin-dependent miRNA in TSC2-deficient cells.

<p>TSC2−/− cells were treated with Rapamycin 20 nM or DMSO for 24 hr and miRNA expression was assessed by qRT-PCR. <b>A</b>) miRNA expression is similar in 621-101 cells using RNU44 (left panel) or RNU48 (right panel) for normalization. <b>B</b>) miRNA expression in 621-101 cells normalized to RNU44. Highlighted results are significant using a Bonferroni correction.