Bloodstream Infection among Adults in Phnom Penh, Cambodia: Key Pathogens and Resistance Patterns

<div><p>Background</p><p>Bloodstream infections (BSI) cause important morbidity and mortality worldwide. In Cambodia, no surveillance data on BSI are available so far.</p> <p>Methods</p><p>From all adults presenting with SIRS at Sihanouk Hospital Centre of HOPE (July 2007–December 2010), 20 ml blood was cultured. Isolates were identified using standard microbiological techniques; antibiotic susceptibilities were assessed using disk diffusion and MicroScan®, with additional E-test, D-test and double disk test where applicable, according to CLSI guidelines.</p> <p>Results</p><p>A total of 5714 samples from 4833 adult patients yielded 501 clinically significant organisms (8.8%) of which 445 available for further analysis. The patients’ median age was 45 years (range 15–99 y), 52.7% were women. HIV-infection and diabetes were present in 15.6% and 8.8% of patients respectively. The overall mortality was 22.5%. Key pathogens included <i>Escherichia coli</i> (n = 132; 29.7%), <i>Salmonella</i> spp. (n = 64; 14.4%), <i>Burkholderia pseudomallei</i> (n = 56; 12.6%) and <i>Staphylococcus aureus</i> (n = 53; 11.9%). Methicillin resistance was seen in 10/46 (21.7%) <i>S. aureus</i>; 4 of them were co-resistant to erythromycin, clindamycin, moxifloxacin and sulphamethoxazole-trimethoprim (SMX-TMP). We noted combined resistance to amoxicillin, SMX-TMP and ciprofloxacin in 81 <i>E. coli</i> isolates (62.3%); 62 isolates (47.7%) were confirmed as producers of extended spectrum beta-lactamase. <i>Salmonella</i> isolates displayed high rates of multidrug resistance (71.2%) with high rates of decreased ciprofloxacin susceptibility (90.0%) in <i>Salmonella</i> Typhi while carbapenem resistance was observed in 5.0% of 20 <i>Acinetobacter</i> sp. isolates.</p> <p>Conclusions</p><p>BSI in Cambodian adults is mainly caused by difficult-to-treat pathogens. These data urge for microbiological capacity building, nationwide surveillance and solid interventions to contain antibiotic resistance.</p>