Motor activity and function.

<p>Open field (OFT), rotarod, and wire hang tests (WHT) were performed on WT and A53T mice at 2–12 months of age (n = 14–18 per group) to analyze motor activity and function. (A) Total distance traveled on the OFT was measured over a ten min period by automated video tracking using ANY-maze software. (B) Latency to fall on the rotarod was recorded by a blinded observer in nine trials performed over three days. (C) Latency to fall from the inverted grid on the WHT was recorded by automated video tracking using ANY-maze software. (D) Heat map occupancy plots from the OFT and WHT were averaged from recorded behavior of all animals in each group. Results are presented as mean ± SEM and were analyzed by two-way ANOVA with Bonferroni post-hoc tests comparing each A53T group to age-matched controls (**p<0.01; ***p<0.001).