Cisplatin resistant HCT116 clones display a prolonged 4N arrest in response to cisplatin.

<p><b>A)</b> HCT116 clones that are relatively cisplatin sensitive (D3, D8) or relatively cisplatin resistant (D6, D7) were untreated (NT) or treated with 15 µM cisplatin for 1–3 days. Cell cycle profiles were determined by flow cytometry. <b>B)</b> The percentage of sub-G1, 2N, S-phase, 4N, or greater than 4N cells was determined at the indicated time points in cisplatin treated cells. Numbers represent the average of 3 separate experiments +/− s.e.m.