<p>A) The regions upstream of <i>clpP1</i> or <i>clpP2</i> tested for promoter activity are marked. B) P<sub>125</sub> activity in <i>M. tuberculosis</i>. C) P<i><sub>clpP2</sub></i> activity in <i>M. tuberculosis</i>. Promoter activity was measured in transformants grown to late exponential phase in standing liquid cultures. Results are the average activity ± standard deviation of three independent transformants assayed in duplicate. Activity is given in Miller Units (MU)- measured as nmol of O-nitrophenol produced per min per mg of protein. Control = pSM128 empty vector control.</p
We have constructed a promoter selection vector for mycobacteria to analyze the sequences involved i...
<p>Promoter activity of seven <i>rrn</i> operons was determined using a series of pGRS vector, two f...
Caseinolytic (Clp) proteases are the most widespread energy-dependent proteases in bacteria. They ar...
<p>A) Sequence of the region upstream of <i>clpP1P2</i>. The <i>tig</i> stop codon, −10 promoter ele...
<p>A to D) <i>M. tuberculosis</i> transformants were grown to late exponential phase in standing liq...
<p>A) <i>M. tuberculosis</i> transformants harbouring P<sub>278</sub> were grown in aerobic culture....
<p>A) P<sub>278</sub> promoter activity in standing liquid cultures. Treatments were: 50 µg/mL of ch...
<p>A) Chromosomal organisation of <i>clpP1</i> and <i>clpP2</i>. Regions amplified for RT-PCR are ma...
<p>(A) Schematic representation of mycobacterial recombineering, employed to replace the endogenous ...
<p>A. DNA sequence of the P<sub>Rv0560c</sub> region. The predicted translation start site of Rv0560...
<p>Promoter activity was measured in <i>M. tuberculosis</i> transformants grown under aerobic growth...
PMCID: PMC3613350This is an open-access article distributed under the terms of the Creative Commons ...
<p>Promoter activity was assayed in the hypoxia model using (A) LacZ and (B) mCherry as a reporter. ...
<p>The -35, -10 elements and the transcription start sites are underlined. Sequences are aligned wit...
Mycobacterium tuberculosis (Mtb) contains two clpP genes, both of which are essential for viability....
We have constructed a promoter selection vector for mycobacteria to analyze the sequences involved i...
<p>Promoter activity of seven <i>rrn</i> operons was determined using a series of pGRS vector, two f...
Caseinolytic (Clp) proteases are the most widespread energy-dependent proteases in bacteria. They ar...
<p>A) Sequence of the region upstream of <i>clpP1P2</i>. The <i>tig</i> stop codon, −10 promoter ele...
<p>A to D) <i>M. tuberculosis</i> transformants were grown to late exponential phase in standing liq...
<p>A) <i>M. tuberculosis</i> transformants harbouring P<sub>278</sub> were grown in aerobic culture....
<p>A) P<sub>278</sub> promoter activity in standing liquid cultures. Treatments were: 50 µg/mL of ch...
<p>A) Chromosomal organisation of <i>clpP1</i> and <i>clpP2</i>. Regions amplified for RT-PCR are ma...
<p>(A) Schematic representation of mycobacterial recombineering, employed to replace the endogenous ...
<p>A. DNA sequence of the P<sub>Rv0560c</sub> region. The predicted translation start site of Rv0560...
<p>Promoter activity was measured in <i>M. tuberculosis</i> transformants grown under aerobic growth...
PMCID: PMC3613350This is an open-access article distributed under the terms of the Creative Commons ...
<p>Promoter activity was assayed in the hypoxia model using (A) LacZ and (B) mCherry as a reporter. ...
<p>The -35, -10 elements and the transcription start sites are underlined. Sequences are aligned wit...
Mycobacterium tuberculosis (Mtb) contains two clpP genes, both of which are essential for viability....
We have constructed a promoter selection vector for mycobacteria to analyze the sequences involved i...
<p>Promoter activity of seven <i>rrn</i> operons was determined using a series of pGRS vector, two f...
Caseinolytic (Clp) proteases are the most widespread energy-dependent proteases in bacteria. They ar...