Analytical size-exclusion chromatography experiments for MjTX-I.

Guilherme H. M. Salvador (397994)
Carlos A. H. Fernandes (397995)
Angelo J. Magro (397996)
Daniela P. Marchi-Salvador (397997)
Walter L. G. Cavalcante (397998)
Roberto M. Fernandez (397999)
Márcia Gallacci (336895)
Andreimar M. Soares (336893)
Cristiano L. P. Oliveira (198163)
Marcos R. M. Fontes (336896)

Publication date

April 2013

DOI

10.1371/journal.pone.0060610.g003

Abstract

<p>(A) The inset shows the 13% SDS-PAGE of MjTX-I collected in different positions of the obtained peak. Lane I: unstained SDS-PAGE Standard from Bio-Rad Laboratories, Inc.; Lane II: MjTX-I collected at the elution volumes correspondent to points 1 and 2 in the chromatogram; Lane III: MjTX-I collected at the elution volume correspondent to point 3 in the chromatogram. (B) Calibration curve obtained using standard proteins shows the different oligomeric assemblies of MjTX-I in 20 mM Tris-HCl pH 8.0. The protein standards were obtained from a low molecular weight gel filtration calibration kit (Sigma-Aldrich) containing: blue dextran (2000 kDa), bovine serum albumin (66 kDa), carbonic anhydrase (29 kDa), cytochrome C (12.4 kDa) and aprotinin ...