Interaction of LATS1 and WWP1 <i>in vivo</i>.

<p>A, B) Co-IP of ectopically expressed LATS1 and WWP1 <i>in vivo</i>. COS7 cell lysates expressing either LATS1-FLAG (A) or WWP1-myc (B) alone or together with WWP1-myc (A) or LATS1-FLAG (B) were immunoprecipitated with anti-Myc (A) or anti-FLAG (B) antibody, followed by western blotting with anti-FLAG antibody (A) or anti-Myc (B) antibody, respectively. Expression of LATS1 levels in cell lysate was also examined by western blot (A, B). C) Co-IP of endogenous LATS1 and WWP1 <i>in vivo</i>. About 1 mg of cell lysates from HEK293T were immunoprecipitated with either control pre-immune serum or rabbit anti-LATS1 (Y03) polyclonal antibody, followed by western blotting with anti-WWP1 antibody. Expression of endogenous WWP1 and LATS1 is also shown.