Expression analysis of the mouse PRAMEL1 protein in testes.

<p><b>a.</b> Western blot analysis of PRAMEL1. Protein extracts from adult mouse testis were subjected to western blot analysis using a mouse PRAMEL1 antibody. An immune-reactive protein with an expected molecular weight of ∼ 57 kDa was detected in the testis, and a very minor band of ∼ 42 kDa was also observed. As a positive and loading control a monoclonal ACTB antibody was used, and an expected immune-reactive protein of 42 kDa was observed. As a negative control, we performed a pre-absorption control by the peptide used to generate the PRAMEL1 antibody, and no band was detected. <b>b.</b> Time course analysis of the PRAMEL1 protein expression during testis development. Protein extracts from newborn (Nb), 1-week (1w), 2-weeks (2w), 3-weeks (3w), 4-weeks (4w) and 8-weeks (8w)-old testes were subjected to western blotting with the mouse PRAMEL1 antibody. The expression level of the PRAMEL1 was gradually increased from Nb to 3w and then remained constant after 3-weeks of age. Numbers on the left indicate the molecular weights of standard proteins.