Inhibition of mitochondrial fission eliminates the glucose stimulation-induced reversible change of mitochondrial morphology.

<p>(A, B) Confocal images of mitochondria in resting (A) and glucose stimulated (B) conditions show long tubular morphology. Scale bar, 5 µm. (C) Cell counting showed that glucose stimulation did not change mitochondrial morphology when fission was inhibited (Ad-DLP1-K38A) whereas glucose stimulation-induced mitochondrial shortening was observed in control cells (Ad-LacZ). (D) Morphometric analyses show that fission inhibition prevented mitochondrial shortening upon glucose stimulation (Ad-DLP1-K38A). Error bars are SEM. n = 7 for Ad-LacZ resting and glucose, and Ad-DLP1-K38A resting, and n = 9 for Ad-DLP1-K38A glucose stimulation. *, <i>P</i><0.05. **, <i>P</i><0.01.