CG0009 does not change AKT activities.

<p>(A) MCF7 cells were treated with 1 µmol/L CG0009 and analyzed by immunoblotting with anti-pAKT^ser473 and AKT antibodies. A densitometric analysis of pAKT^ser473 was done with Quantity One software (BIO-RAD). (B) MCF7 cells were treated with 1 µmol/L CG0009 for the indicated times and whole cell lysates were prepared for <i>in vitro</i> AKT kinase assay. Purified recombinant AKT proteins were used as a positive control. Columns represent means of three independent experiments; <i>bars</i> denote SD (* <i>P</i><0.05).