TL1A does not affect B-cell survival.

<p>Purified B cells were activated with 2 µg/ml anti-IgM, 20 U/ml IL-2 for 24, 48,72 and 96 h, in presence or absence of 100 ng/ml TL1A, stained with annexinV/PI and analyzed by flow cytometry. Analyses were gated on CD19-positive cells. (<b>A</b>) Dot plots representatives of three independent experiments. Bold numbers indicate percentage of cells in each quadrant. (<b>B</b>) Percentage of viable cells (annexinV-negative/PI-negative) following incubation with 2 µg/ml anti-IgM, 20 U/ml IL-2; 2 µg/ml anti-IgM, 20 U/ml IL-2, 100 ng/ml TL1A; medium. Data are represented as mean±SEM. * = <i>p</i><0.05; ns = not significant.