Except for Per2 in ARP and FRP, clock genes in the liver follow food and activity.

<p>Relative mRNA temporal profiles of clock genes along the light/dark (LD) cycle were examined in the liver of control. (n = 3 rats per time point). A) Black and white bars at the bottom of the figure represent the LD cycle; the striped bars represent the time spent in the slow rotating wheel for the ARP an AAP rats respectively. B) The striped bar indicates the light period in which FRP animals received their food. Quantification of genes was normalized to Actin. Daily curves for clock and metabolic genes were tested with a cosinor analysis to determine significance in amplitude and to determine the acrophase. See also <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060052#pone-0060052-t002" target="_blank">table 2</a> for details. Asterisk indicates a significant rhythm in the CTRL group, † indicates a significant rhythm in the ARP group, # indicates a significant rhythm in the FRP group,+indicates a significant rhythm in the AAP group and ‡ indicates significant rhythm in the FAP group. These results were corroborated with Kruskall-Wallis test (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060052#pone-0060052-t003" target="_blank">Table 3</a>).