<p>(A) Mtb responds synergistically to Cl<sup>−</sup> and pH. CDC1551(<i>rv2390c</i>'::GFP) was grown <i>in vitro</i> in media at pH 7.0 (control, circles), pH 7.0+250 mM NaCl (squares), pH 5.7 (triangles), or pH 5.7+250 mM NaCl (diamonds). Samples were taken over time, fixed, and GFP signal analyzed by FACS. Data are shown as means ± SD from 3 independent experiments. (B) Mtb's synergistic response to Cl<sup>−</sup> and pH is reflected transcriptionally. qRT-PCR of gene expression in WT grown as in (A) for 4 hrs. Fold induction is as compared to WT grown in media at pH 7.0. Data are shown as means ± SD from 3 technical replicates. (C) <i>phoPR</i> is required for Mtb's response to pH and plays a role in its Cl<sup>−</sup> response. CDC1551...
<p><b>A</b>, Increases in absorbance of the reactions with <i>Tc</i>CYS4 (+<i>Tc</i>CYS4) and withou...
<p>The strains used were WT (STH2370 wild type), Δ<i>phoP</i> (<i>phoP</i>::<i>cam</i>), Δ<i>phoP</i...
<p>A) Estimation of intracellular cAMP levels in Mtb. Wild-type Mtb CDC1551 was cultured in Middlebr...
(A) rv0500A disruption enhances Mtb response to acidic pH and high [Cl-]. WT, rv0500A::Tn, or rv0500...
<p>(A) Mtb responds to Cl<sup>−</sup> and pH. CDC1551(<i>rv2390c</i>'::GFP) was grown <i>in vitro</i...
(A) Mtb ΔceoBC mutant growth during environmental stress. WT or ΔceoBC mutant Mtb were grown in stan...
PubMed ID: 23592993This is an open-access article distributed under the terms of the Creative Common...
(A) Mtb response to K+ is concentration-dependent. CDC1551(kdpF’::GFP, smyc’::mCherry) was grown in ...
<p>(A and B) Expression of <i>rv2390c-rpfD</i> is upregulated during Mtb infection of activated vs. ...
<p>Survival kinetics of AS-recombinants: (A) <i>rpoB</i>, (B) <i>rpoC</i>, (C) <i>ppk</i>, (D) <i>ar...
(A and B) qRT-PCR of gene expression in WT grown in K+-free 7H9, pH 7, compared to standard 7H9, pH ...
<p>(A and B) Greater induction of <i>rv2390c</i>'::GFP during Mtb infection of WT vs. IFNγ<sup>−/−</...
<p>The central three coloured contour plots show the influences of the combined stresses of NaCl and...
<p>Accessions with potentially functional (Bay-0, Col-0) and defective <i>MOT1</i> alleles (L<i>er</...
<p>(<b>A</b>) <i>M</i>. <i>tuberculosis</i> WT, Δ<i>pstS1</i>, and Δ<i>pstS1</i> pMV<i>pstS1</i> wer...
<p><b>A</b>, Increases in absorbance of the reactions with <i>Tc</i>CYS4 (+<i>Tc</i>CYS4) and withou...
<p>The strains used were WT (STH2370 wild type), Δ<i>phoP</i> (<i>phoP</i>::<i>cam</i>), Δ<i>phoP</i...
<p>A) Estimation of intracellular cAMP levels in Mtb. Wild-type Mtb CDC1551 was cultured in Middlebr...
(A) rv0500A disruption enhances Mtb response to acidic pH and high [Cl-]. WT, rv0500A::Tn, or rv0500...
<p>(A) Mtb responds to Cl<sup>−</sup> and pH. CDC1551(<i>rv2390c</i>'::GFP) was grown <i>in vitro</i...
(A) Mtb ΔceoBC mutant growth during environmental stress. WT or ΔceoBC mutant Mtb were grown in stan...
PubMed ID: 23592993This is an open-access article distributed under the terms of the Creative Common...
(A) Mtb response to K+ is concentration-dependent. CDC1551(kdpF’::GFP, smyc’::mCherry) was grown in ...
<p>(A and B) Expression of <i>rv2390c-rpfD</i> is upregulated during Mtb infection of activated vs. ...
<p>Survival kinetics of AS-recombinants: (A) <i>rpoB</i>, (B) <i>rpoC</i>, (C) <i>ppk</i>, (D) <i>ar...
(A and B) qRT-PCR of gene expression in WT grown in K+-free 7H9, pH 7, compared to standard 7H9, pH ...
<p>(A and B) Greater induction of <i>rv2390c</i>'::GFP during Mtb infection of WT vs. IFNγ<sup>−/−</...
<p>The central three coloured contour plots show the influences of the combined stresses of NaCl and...
<p>Accessions with potentially functional (Bay-0, Col-0) and defective <i>MOT1</i> alleles (L<i>er</...
<p>(<b>A</b>) <i>M</i>. <i>tuberculosis</i> WT, Δ<i>pstS1</i>, and Δ<i>pstS1</i> pMV<i>pstS1</i> wer...
<p><b>A</b>, Increases in absorbance of the reactions with <i>Tc</i>CYS4 (+<i>Tc</i>CYS4) and withou...
<p>The strains used were WT (STH2370 wild type), Δ<i>phoP</i> (<i>phoP</i>::<i>cam</i>), Δ<i>phoP</i...
<p>A) Estimation of intracellular cAMP levels in Mtb. Wild-type Mtb CDC1551 was cultured in Middlebr...