Luciferase signal and MHV-68 antigen detection in isolated male genital tract after sexual transmission.

<p><b>A.</b> A mouse equivalent to that in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003292#ppat-1003292-g007" target="_blank">Figure 7</a> was dissected and its genital tract imaged <i>ex vivo</i> at day 10 post-contact with the infected female. The images are representative of data from at least 5 mice, and show either a standard photograph (Photo) or that photograph overlaid with the luciferase signal (Photo+Luc). The region with the highest signal was isolated and processed for histological analysis. The scale bar (photons sec⁻¹ cm⁻² steradian⁻¹) shows the color scheme for signal intensity. MUMP, male urogenital mating protuberance. <b>B.</b> The piece of penis isolated in A. was fixed in formaldehyde and organ slices were either stained with hematoxylin-eosin (panel i) or processed for immunohistochemistry with anti-MHV-68 rabbit polyserum (panels ii to v) as described in the <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003292#s4" target="_blank">Materials and Methods</a> section. Rectangles indicate regions highlighted in the following panels. Filled and open arrows indicate detection of MHV-68 antigens in superficial regions of the penis and in deeper region of the Corpus cavernosum, respectively. Ur, urethra; EpPe, Epithelium of the penis; BoMa, Bone marrow; OsPe, Os penis; CoCa, Corpus cavernosum; FiPa, Filiform papilla.