Evaluation of BP-induced expression of several endogenous estrogen-regulated genes.

<p>After hormone deprivation for 24 h, MCF-7 cells were grown in medium containing 2.5% dextran-treated charcoal stripped FBS and treated with 10⁻⁸ M E2 or different concentrations of BPs (10⁻⁸, 10⁻⁷ and 10⁻⁶ M, illustrated by color gradations) for 48 h. In addition, treatments to 10⁻⁶ M BPs were also performed in presence of 10⁻⁷ M of the anti-estrogen ICI_182,780 (ICI) (open bars for BP + ICI treatments and hatched bar for E2 + ICI treatment). The expression levels of several E2-regulated genes, (<b>A</b>) CXCL12, (<b>B</b>) Amphiregulin, (<b>C</b>) pS2 and (<b>D</b>) Progesterone Receptor (PR), after the treatments were quantified using real-time PCR. Data are the mean values from triplicate experiments ± SEM (* P<0.05, **P<0.01, ***P<0.001).