Kinetics of the production of inflammatory cytokines IFNγ and TNFα after chemotherapy based conditioning and transplantation with syngeneic or allogeneic bone marrow and splenic cells.

<p>Female BALB/c mice were conditioned using a Bu-Cy regimen and transplanted with bone marrow and spleen cells in an allogeneic or syngeneic setting (see Methods and materials). Blood samples were collected at different time points prior to and after transplantation (D-7, D0, D+5 and D+7), and serum was separated. Thereafter, serum levels of IFNγ and TNFα were measured employing ELISA techniques. *Statistically different (p≤0.05) from the control value (no treatment with Bu-Cy or transplantation, D-7) as determined by the ANOVA test. ¤ Statistically different (p≤0.05) from the syngeneic-transplanted mice as determined by the ANOVA test.