Inhibition of p38 MAPK affects TJP1 localization after 12 h and 24h.

<p>TJP1 protein localization following treatment with KSOMaa, SB 202474, or SB 220025. Representative images of TJP1 after 12 h (A) and 24 h (B) exposure. SB 220025 treatment affected TJP1 localization after 12 h (A) and 24 h (B). In KSOMaa and SB 202474 images, white arrows indicate normal localization of TJP1 along adjacent cell borders. In p38 MAPK inhibited images, white arrows indicate punctate distribution of TJP1 compared to control embryos. In control embryos, yellow staining indicates co-localization of TJP1 and F-actin (yellow arrowhead). In treated embryos, there is reduced yellow staining, and increased red staining indicating a loss of co-localization of TJP1 and F-actin (yellow arrowhead). Blue = nucleus; Red = F-actin; Green = TJP1; <i>n</i> = 10–15 embryos in each group; scale bar = 10 microns.