<i>Cryptococcus</i> cells penetrate into the brain by a mechanism involving fungal inositol transporters.

<p>Confocal analyses were performed using tissue sections obtained from mice that were either uninfected, or infected with wild type H99 (WT) or the <i>itr1aΔ itr3cΔ</i> mutant for 48 hr. Lesions were identified by the high accumulation of GXM within the CNS parenchyma (<i>Cn</i>, green channel) and DAPI staining (blue channel) to observe the nuclei in the cells. Animal brains infected with the mutant have less and smaller lesions. Bar indicates 75 µm.