The combination of CpG and R848 inhibits the induction of CD4+CD25+ Tregs in pVAX1-Sj26GST–vaccinated mice.

<p>Seven days after the last immunization with pVAX1-Sj26GST, alone or with CpG, R848, or both (CpG+R848), splenocytes from each mouse were analyzed by flow cytometry analysis for CD3, CD4, CD25, and Foxp3 expression. The mean fold change of the percentage of CD4⁺CD25⁺Foxp3⁺ Tregs in mice at week 0 compared with 5 weeks after immunization with the indicated formulations is shown. Groups consisted of 6 mice at each time point. The results are expressed as the mean ± SEM of 12 mice from 2 independent experiments. ** <i>P</i><0.01.