CD4⁺CD25^hi Treg cells induced by IgG-NK cells suppresses CD4⁺ T cells proliferation.

<p>(<b>A, B</b>) CD4⁺CD25^hi Treg cells was induced by coculturing IgG-NK cells and CD4⁺CD62L⁺ T cells and sorted by FACS. 2×10⁴ CFSE-labeled CD4⁺CD62L⁺ T cells were cocultured with the sorted CD4⁺CD25^hi T cells according to the indicated ratios. Plate bound anti-CD3 and soluble anti-CD28 antibodies were used to stimulate T cell proliferation. The intensities of CFSE were determined at day 4 by flow cytometry. CD4⁺CD25^hi Treg cells significantly suppressed CD4⁺ T cells proliferation at 1 to 1 ratio (p<0.01). Representative of 4 independent experiments (<b>A</b>); Data pooled from 4 independent experiments (<b>B</b>).