Polymerization of FtsZ mutants in vitro is not affected by MinC.

<p>A. Representative light scattering trace of an experiment performed with wild-type FtsZ in the absence of inhibitor and in the presence of MinC or MinC19. Reactions contained 7 µM of FtsZ and 20 µM of MinC or MinC19 in Mes/NaOH 50 mM, MgCl₂ 10 mM, KCl 133 mM, DEAE-dextran 0.6 mg/mL, pH 6.5. B. Experiments similar to A were performed and the inhibition of the polymerization of each FtsZ mutant by MinC was quantified relative to the maximum light scattering signal in the absence of MinC. The original light scattering traces corresponding to this graph can be found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060690#pone.0060690.s004" target="_blank">Fig. S4</a>. Measurements were repeated at least three times for each FtsZ mutant with similar results.