Co-localization of virus particles with endosomal markers.

<p>Cells were incubated with fluorescently labeled virus particles (green) for 3 h. Cells were either untreated or pretreated with 50 µM CQ for 1 h before addition of and then during incubation with virus. After the incubation period cells were fixed and stained with EEA1 or LAMP1 reactive antibodies and corresponding secondary antibody (red). Cells were then stained with DAPI to visualize cell nuclei (blue) and were imaged by confocal microscopy. Arrowheads indicate representative virus particles co-localized with each endosomal marker.