Shark sdAb also bind the NP C-terminal region and are able to form MARSAs.

<p>A) Confirming shark sdAb bound the <i>E. coli</i> rec-NP preparations as sdAb-AP fusions [Bundibugyo (B), Ivory Coast (IC), Reston (R), Sudan (S), Zaire (R), and negative control Marburg (M)] before, B) identifying the C-terminal domain was the major antigenic determinant by probing on the <i>E. coli</i> expressed Zaire rec-NP deletion mutants (notation of deletions as for <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061232#pone-0061232-g005" target="_blank">figure 5</a>, with #7 the C-terminal deletion mutant). C) Confirming shark sdAb bound our recombinant HEK 293T preparations as sdAb-AP fusions by western blot before, D) employing them both in the AP based MARSA to reveal that shark sdAb can indeed form a monoclonal capture ELISA, though they appear less sensitive than the positive control llama anti-Zaire E sdAb for Zaire, Reston and Sudan rec-NP preparations. E) Employing 1e+4 pfu/well of virus in the sandwich assay to reveal the shark clones can bind Ivory Coast in a MARSA format.