CD4+ T cell subsets in lymph nodes and prostates of CP-1-infected mice.

<p>(A) Representative flow cytometry analysis of CD4+ T cells from lymph nodes of naïve and CP-1 infected NOD and B6 mice. Isolated lymph node cells were gated on CD4+ T cells followed by IFN-γ+, IL-17A+, IFN-γ+ IL-17A+, and IL-4+ expression. (B) Representative flow cytometry analysis of intraprostatic CD4+ T cells from naïve or CP-1 infected NOD and B6 mice. Histograms representing intracellular cytokine staining in CP-1 infected (solid line) NOD or B6 mice were overlaid with naïve T cells (tinted) and isotype controls (dotted line). Each experiment was independently repeated at least three times with 5 mice/group. In real-time PCR assays, prostates from NOD mice infected with CP1 were used to prepare cDNA and real-time PCR was performed for lineage-specific transcription factors Tbet, Foxp3, RORγt, as well as CD4. Expression levels are shown as Ct(TF)/Ct(CD4) and is the average of three independent experiments.