Effect of honokiol on PGE₂ production and PGE₂-enhanced migration of NSCLC cells.

<p>(<b>A</b>) Treatment of A549 and H1299 cells with honokiol reduced the levels of PGE₂ in a dose-dependent manner. The levels of PGE₂ were measured in cell lysates using PGE₂ immunoassay kit and results are expressed in terms of pg/mg protein ± SD, n = 3. Significant reduction versus control cells, *<i>P</i><0.001, ^†<i>P</i><0.01. (<b>B</b>) Treatment of A549 and H1299 cells with honokiol inhibits PGE₂-enhanced cell migration ability. The data on cell migration capacity of cells are summarized as a mean number of migratory cells ± SD/microscopic field, n = 2. Significant inhibition versus PGE₂ alone treatment; *<i>P</i><0.001, ^†<i>P</i><0.01.