Honokiol inhibits the activation of NF-κB/p65 in NSCLC cells.

<p>(<b>A</b>) A549 and H1299 cells were treated with varying concentrations of honokiol for 24 h, cells were harvested and cytosolic and nuclear fractions were subjected to western blot analysis. (<b>B</b>) The activity of NF-κB/p65 in the nuclear fractions of cells were measured using NF-κB/p65-specific activity assay kit, n = 3. NF-κB/p65 activity is expressed in terms of percent of non-honokiol-treated control cells. Significant inhibition <i>versus</i> control, *<i>P</i><0.001, ^¶<i>P</i><0.05. (<b>C</b>) Treatment of cells with CAPE, an inhibitor of NF-κB, for 24 h inhibits the migration of cells in a dose-dependent manner. (<b>D</b>) Data on cell migration are summarized as the mean number of migrating cells ± SD per microscopic field/group. Significant inhibition <i>versus</i> control group, *<i>P</i><0.001, ^¶<i>P</i><0.05. (<b>E</b>) Treatment of cells with CAPE for 24 h inhibits the levels of NF-κB and IKKα as determined using western blot analysis.