Protection of mice from challenge with live RSV.

<p>BALB/c mice were immunized IN with RSV-virosomal vaccine formulations (5 µg of protein) or HNE. Control mouse groups were either immunized IM with FI-RSV or IN with L-RSV on day 0 and 21. Mice were challenged with live-RSV on day 28 and four days after challenge (day 32), lung viral titers were determined. Data from 6 mice per group is shown. Viral titers are expressed as TCID₅₀. Bars and error bars represent means ± SD. The data shown are representative of at least 2 separate experiments. Data was analyzed by a Mann-Whitney U test and a p-value of ≤ 0.05 was considered to represent a significant difference. Asterisks indicate groups that had significantly lower viral titers compared to titers in the non-immune HNE group. Horizontal lines compares differences in titers in different immunized groups. * p≤0.05, ** p≤0.01.