Purification of recombinant HP-NAP from <i>B. subtilis</i> by DEAE Sephadex anion-exchange chromatography.

<p>A, The soluble fraction from the whole cell lysate of <i>B. subtilis</i> DB104-pRPA-NAP was applied to DEAE Sephadex anion-exchange column as described in Materials and Methods. The chromatogram was recorded by UV absorbance at 280 nm. The fractions of flow-through, wash, and elution were ranged from fractions 1 to 22, 23 to 42, and 43 to 63, respectively. The inset represents the enlarged chromatogram of fractions 1 to 37. B, The whole cell lysate (W), soluble fraction (S), and insoluble pellet (I) of <i>B. subtilis</i> DB104-pRPA-NAP and the selected fractions corresponding to the fraction number of chromatogram shown in (<b>A</b>) were analyzed by SDS-PAGE. Molecular weights (M) in kDa are indicated on the left of the stained gels.