MPO-dependent increase of MPO levels are attenuated by resveratrol in rat primary microglia and astrocytes.

<p><b>A.</b> Rat primary microglia (PM, upper) and astrocytes (PA, lower) were pretreated with resveratrol (RESV), ethyl pyruvate (EtPy), or 15d-PGJ2 for 1 h, followed by incubation with 100 ng/ml MPO for 18 h. MPO levels were determined by Western blot analyses. <b>B.</b> Concentration-dependent effects of resveratrol were observed in MPO-treated primary microglia. <b>C.</b> Rat primary microglia were treated with 20 µM resveratrol and/or 100 ng/ml MPO at various time points. The cells were further incubated for 18 h and then MPO levels were examined by western blot analyses. The data shown are representative of at least three independent experiments.