Wnt signaling is dysregulated in <i>inv^−/−</i> MEFs.

<p>(<b>A</b>) WB analysis of growth arrested <i>inv^+/+</i> and <i>inv^−/−</i> MEFs with antibodies against Dishevelled (Dvl) -1, -2 and -3, Adenomatous polyposis coli (APC), Glycogen synthethase kinase 3 β (GSK3β), β-catenin (β-cat) and S⁴⁵-phosphorylated β-catenin (p-β-cat). (<b>B</b>) Quantifications of WB analysis from (A). Histograms represent mean ± S.E.M. (n≥3). (<b>C,G</b>) IFM analysis of growth arrested <i>inv^+/+</i> and <i>inv^−/−</i> MEFs. Primary cilia (Ac-tub or Glu-tub, <i>red</i>) are marked with closed arrows, nuclei are stained with DAPI (<i>blue</i>). <i>Green</i> protein localizations are Dvl-1 (C), Dvl-2 (D), Dvl-3 (E), p-β-cat (F) and Frizzled-3 (Fzd-3) (G). (<b>H,I</b>) IFM analysis on the localization of S^33/37-T⁴¹-phosphorylated β-catenin (p-β-cat) (<i>blue</i>, open arrows) to the ciliary base (asterisks) in mock (H) and Inv::GFP (<i>green</i>) (I) transfected <i>inv^+/+</i> and <i>inv^−/−</i> MEFs. Primary cilia (Glu-tub, <i>red</i>) are marked with closed arrows.