Gene redundancy for cobalamin and tetrahydrofolate metabolism in <i>M. extorquens</i> CM4.

a<p>Homologs with >90% aa Id (with mentioned exceptions) found in the chromosome of all <i>M. extorquens</i> strains AM1, BJ001, DM4, and PA1 (common core genome), in one of the strains (shared accessory genome), or none of these strains (CM4 specific CDS). The accessory genome includes a <i>btuB</i> homolog (Mpop_3807, 65% aa Id) in strain BJ001. For strain AM1, a putative dihydrofolate reductase <i>dfrB</i> gene (META2_0242, 34 and 28% aa Id with DmrA and DfrA, respectively) is found in addition to the chromosomal gene; moreover, homologs to Mchl_1923 (META2_0462, 33% aa Id with the N-terminal domain), and CzcA2 (META2_1026, 85% aa Id with pCMU01 plasmid <i>czcA2</i>) are found.</p>b<p>MaGe annotation (<a href="https://www.genoscope.cns.fr/agc/microscope" target="_blank">https://www.genoscope.cns.fr/agc/microscope</a>).</p>c<p>Precursors are uroporphyrinogen III and 5,6-dimethylbenzimidazole.</p>d<p>n.d., not detected.</p>e<p>Encode for homologs of different length: CobA (267 aa)/CysG (485 aa); CobC2 (519 aa)/CobC (338 aa); PurU (287 aa)/PurN (219 aa).</p>f<p>In <i>M. extorquens</i> strains, H₄F is synthesized either <i>de novo</i> or salvaged from 5,10-methenyl-H₄F, or 5- or 10-formyl-H₄F <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0056598#pone.0056598-Vuilleumier2" target="_blank">[11]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0056598#pone.0056598-Maden1" target="_blank">[72]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0056598#pone.0056598-Vorholt1" target="_blank">[73]</a>.