ScFv binding activity as determined using a novel homogeneous bridging electrochemiluminescent (MSD) assay.

<p><b>A</b> Biotinylated scFv immobilized onto streptavidin-coated microtiter wells were used to capture autoantibodies. Captured autoantibodies were detected using Sulo-Tag labeled scFv and an MSD plate reader. <b>B</b> Binding activity of scFv antibody with autoantibodies obtained from stage I lung adenocarcinoma (AdI, n = 14) and from non-cancer (C, n = 21) human serum samples. Each data point represents the assay signal intensity for individual serum samples. P = 0.031 for normal (C) vs cancer (AdI) group average (horizontal lines).