Binding of calreticulin to the synthetic peptide containing the γ-cleavage site of APP.

<p>Recombinant calreticulin (<b>A</b>) or synthetic biotinylated peptides comprising the γ-secretase cleavage site (APP- γ) or the β-secretase cleavage site (APP-β) (<b>B</b>) were coated as substrates and incubated with different concentrations of biotinylated APP-γ or APP-β peptides (<b>A</b>) or recombinant calreticulin (<b>B</b>). Detection of bound proteins was carried out using HRP-coupled streptavidin (<b>A</b>) or the rabbit calreticulin antibody CRT283 and HRP-coupled secondary antibodies (<b>B</b>). Mean values ± SD from three independent experiments are shown.