Effects of PMS1077 and its structural analogs on TNF-α induced NF-κB activation.

<p>(A) DU145-NF-κB-Luciferase cells were treated with PMS1077 and its structural analogs at final concentration of 50 µM. TPCA-1 (2 µM) is a specific inhibitor of NF-κB for positive control and DMSO as vehicle. Then cells were left untreated or exposed to TNF-α (20 ng/ml) for 12 h. Luciferase activity was measured using ONE-Glo® Luciferase Assay System (Promega). (B) DU145 cells were treated with PMS601, PMS1077, PMS1120 and PMS1144 at the indicated concentrations for 12 h. Cell viability was determined using MTT assay. The values are the mean±S.D. for three independent replicates. *<i>P</i><0.05 versus TNF-α stimulated group.