Compounds from the kinase inhibitior library are identified as ABCB1 inhibitors by the high-throughput imaging-based efflux assay.

*<p>Compounds that have been previously reported to interact with ABCB1. References are listed separately (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060334#pone.0060334.s004" target="_blank">Text S1</a>).</p><p>KB-V1 cells treated with the kinase inhibitor library of 193 compounds at 10 µM and 1 µM calcein AM were examined by the IncuCyte^TMFLR. The object intensities of each sample were normalized to XR9576 treated cells and expressed as the percentage of inhibition. Compounds that displayed at least 50% inhibition of ABCB1-mediated efflux in all three independent experiments are listed.