Adipose tissue secretion of FNDC5/irisin.

<p>FNDC5/irisin secretion was assessed in adipose tissue secretomes from SAT, VAT and BAT under ad libitum conditions (A); in SAT and VAT secretomes from animals after one and three weeks of exercise training (B); and in SAT, VAT and BAT from animals with 36-h food restriction or re-feed after 15 minutes (C). FNDC5/irisin SAT and VAT secretion from the DIO and Zucker animal models and anorexic animals are also shown (D, E). Irisin circulating levels for all previous situations are shown taking the most intense band at 25 kDa (F). Representative images of western blots and histograms of band quantifications from at least 3 independent experiments are shown. The results are represented as percentages with regard to each control as well as significance. In the situation of 3 weeks of exercise, results are compared with 1 week of exercise (*p<0.05; **p<0.01). SAT: subcutaneous adipose tissue; VAT: visceral adipose tissue; BAT: brown adipose tissue; DIO: diet induced obesity; CDIO: control DIO; CZ (fa/-): control Zucker; Zucker: fa/fa; CONT: control; EXER: exercise; CABA: control ABA; ABA: activity based anorexia.