PMB-specific Ab responses in plasma.

<p><i>A.</i> The PMB-specific Ab titers were determined by ELISA. To establish the ELISA system, 1,000 ng/well of anti-PMB mAb and twofold serial dilutions were tested. Standard anti-PMB was titrated on PMB-coated plates with a sensitivity of 20 ng/well. <i>B.</i> Plasma samples from mice intranasally immunized with OVA plus 0, 5, 50, or 500 µg of PMB 3 times at weekly intervals were assessed by PMB-specific endpoint ELISA. Each dot represents an individual mouse. Horizontal and vertical bars indicate median and IQR, respectively, from 2 independent experiments (with 8 mice in each experimental group).