Luciferase activity of the Py-GFP-luc parasite.

<p>(A, B) Luminescence during erythrocytic stages. (A) Py-GFP-luc infected red blood cells were serially diluted 1∶4 in RPMI and were mixed with an equal volume of luciferin. Luminescence (relative light units, RLU) was measured with a Centro microplate reader. Dashed line indicates background luminescence as determined by measuring uninfected RBCs. (B) Rainbow images of luminescence in BALB/cJ mice with Py-GFP-luc blood stage parasitemia. Mice were injected with luciferin and imaged. Bioluminescence was measured in total flux (p/s). (C, D) Luminescence of Py-GFP-luc oocysts. (C) Midguts from Py-GFP-luc infected mosquitoes were isolated 10 days after an infectious blood meal. The number of oocysts per midgut was estimated and midguts were categorized as follows: Uninfected (0), between 1 and 10 oocysts/midgut (1<10), between 11 and 100 oocysts/midgut (11<100), and greater than 100 oocysts/midgut (>100). Luciferase activity from individual midguts was determined using the Centro microplate reader (RLU) (C) and the IVIS animal imager (total flux) (D). (E) Luminescence of Py-GFP-luc sporozoites. Sporozoites were isolated from salivary glands of infected mosquitoes and serially diluted 1∶10 in RPMI. Luminescence (RLU) was determined as in (A). Dashed line indicates background luminescence of RPMI.