Inhibition of type I BMP receptors in lung cancer cell lines induces cell death.

<p>(<b>A–B</b>) H1299 and A549 cultured in DMEM 5% FCS were treated with DMSO or a BMP receptor antagonist (10 µM Dorsomorphin or 1 µM of selective antagonist). The percentage of cells that take up ethidium bromide was then determined. The data is reported as the mean of at least 3 independent experiments. (<b>C</b>) H1299 cells cultured in DMEM 5% FCS were treated with DMSO, 1 µM and μ5 M of DMH2 for 7 days, stained with Typan Blue, and cell counts performed. Data represents the mean of 4 experiments reported as the percent dead cells. (<b>D</b>) H1299 cells cultured in SFM were treated with DMSO or 1 µM of DMH2 for 7 days, stained with Typan Blue, and cell counts performed. Data represents the mean of 5 experiments reported as percent dead cells. (<b>E</b>) Cell death was determined using flow cytometry detecting uptake of an amine-reactive fluorescent dye in H1299 cells treated with DMS0 or DMH2 for 4 days. Data represent the mean of 3 independent experiments. (<b>F</b>) The H1299 cells were transfected with control siRNA and siRNA targeting alk2, alk3, and alk6. After 2 days the percentage of cells staining for ethidium bromide was determined. The data is reported as the mean of 6 independent experiments.