Down-regulation of CLCA1 expression in CRC.

<p><b>A.</b> Normalized raw expression values of chloride channel family members were analyzed in human colonic mucosa and early CRC tissues from Gene Expression Omnibus (reference series is GSE4017). CLCA1, CLCA4, CLCN2, CLCN3 and CFTR were down-regulated significantly in early CRC patients. Values are mean±s.e.m, *<i>p</i><0.01. <b>B.</b> Analysis of immunofluorescence demonstrated that the expression of CLCA1 was reduced in CRC tissues in contrast to normal colonic mucosa. Bar = 50 µm.