miR-27b expression in cancer stem cells (CSCs) and colorectal cancer (CRC) tumor tissue.

<p>(A) Differentially expressed miRNA in CD133⁺ and CD133⁻ cells. Red denotes high and green denotes low levels of expression. (B) miR-27b expression in CD133⁺ and CD133⁻ cells was assessed by qPCR. The y-axis indicates fold change. (C) miR-27b expression assessed by qPCR in fresh CRC tissues compared to adjacent normal tissues from six patients. The y-axis indicates fold change. (D) miR-27b expression assessed by qPCR in 80 paired paraffin-embedded CRC and adjacent normal tissues. miR-27b levels were normalized to U6 and expressed in terms of the threshold cycle (C_t) ratio. Error bars represent the means ± SEM, *<i>P</i><0.05, **<i>P</i><0.01.