DNA hypermethylation is associated with decreased miR-27b expression.

<p>(A) The levels of miR-27b expression in colorectal cancer (CRC) cell lines were determined by qPCR after treatment with 5AZA or TSA for 3 days. (B) Results of luciferase activity assays following transfection with the predicted miR-27b promoter normalized to pRL-TK Renilla luciferase. (C) MSP analysis of the miR-27b CpG island in a set of CRC cell lines. Bands in the ‘M’ lanes are PCR products obtained using methylation-specific primers and those in the ‘U’ lanes are products obtained using unmethylated-specific primers.