Preincubation with forskolin reduces phosphorylation of cofilin in PCA.

<p>PCA rings were treated with the same conditions as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060986#pone-0060986-g003" target="_blank">Figure 3</a>. Proteins were extracted and separated on SDS polyacrylamide gels and p-cofilin was detected by western blotting. Panel A: Representative western blot of p-cofilin and np-cofilin. Panel B: Cumulative data representing the relative amounts of p-cofilin over the total cofilin and then normalized to the basal. Quantitative values were obtained when the phosphorylated and non-phosphorylated bands were quantitated densitometrically. n = 4, p<0.05. *significant compared to all other conditions.