Effects and duration of transgene expression in baculovirus-infected hUCB-MSCs.

<p><b>A</b>: Cell viability of hUCB-MSCs infected with Bac-NIS at different MOIs at 24 hpi as detected by the CCK-8 assay. <b>B</b>: Cell proliferation of hUCB-MSCs infected by Bac-NIS at different MOIs over time (1, 3, 5, 7, 9, 11, 13 and 15 dpi), as detected by the CCK-8 assay. The cells were seeded 1 day before infection with Bac-NIS and reseeded at 8 dpi when the cells reached ∼80% confluency. <b>C</b>: GFP⁺ % of Bac-GFP-infected hUCB-MSCs over time (1, 2, 3, 4, 8 and 12 dpi). The cells were reseeded at 8 dpi when reached ∼80% confluency. Results are means ± SD (n = 3). <b>Abbreviations</b>: Bac-NIS, recombinant baculovirus carrying sodium-iodide symporter reporter gene; dpi, days post-infection; CCK-8, Cell Counting Kit-8.