DCEF induces the generation of ROS in U87, C6 and U251 glioma cells.

<p>Generation of intracellular ROS was detected by the semi-quantitative technique based on the use of DCFH-DA (A) and HE (B) 15, 30, and 60 minutes after the treatment of cells with 200 mV/mm. Cells without DCEF stimulation were used as the negative control. Generation of intracellular hydrogen peroxide was detected by DCFH-DA (green fluorescence) and superoxide was detected by HE (red fluorescence) 30 minutes after EF treatment with a strength of 200 mV/mm (C). *Compared with the negative control, <i>P</i><0.05; Bar = 50 µm.